ZHANG C X, HE Y X, GUO X M, et al.Community structure variations and driving factors of eukaryotes phytoplankton in Danjiangkou Reservoir in summer and winter[J].Journal of Henan Polytechnic University(Natural Science) ,2022,41(6):110-122.

doi:10.16186/j.cnki.1673-9787.2022050046

Received:2022/05/13

Revised:2022/06/10

Published:2022/11/25

Community structure variations and driving factors of eukaryotes

 phytoplankton in Danjiangkou Reservoir in summer and winter

ZHANG Chunxia1,2, HE Yuxiao1, GUO Xiaoming1, MENG Hongqi1, WU Li1, HUANG Jin3, LI Weiguo1, ZHAO Tongqian1

1.College of Resources and Environment，Henan Polytechnic University，Jiaozuo  454000，Henan，China；2.School of Emergency Management， Henan Polytechnic University，Jiaozuo  454000，Henan，China；3.Ecological Environment Monitoring Center of Qushou in the Middle Route of South-to-North Water Transfer in Henan Province，Nanyang  474450，Henan，China

Abstract:In order to explore the spatio-temporal variation characteristics and driving factors of eukaryotic phytoplankton community structure in Danjiangkou Reservoir，Illumina Miseq high-throughput sequencing technique was used to analyze the phytoplankton community composition and environmental factors in July 2019 and January 2020.The results showed that in summer，7 phyla，134 genera，191 species of phytoplankton were identified，mainly Chlorophyta and Bacillariophyta.The species of Chlorophyta was 2.51 times higher than that of bacillariophyta，but the abundance of bacillariophyta was 2.77 times that of chlorophyta.The average diversity，richness and evenness were 1.58，84.87 and 0.36，respectively.The dominant genera were Discostella of diatom and Botryococcus of chlorophyta.In winter，6 phyla，105 genera，139 species of phytoplankton were identified，the dominant phylum were chlorophyta，followed by bacillariophyta，the species and abundance of chlorophyte were 2.66 times and 2.30 times that of diatom，respectively.The average diversity，richness and evenness were higher than those in summer，which were 2.63，99.56 and 0.58.The main dominant genera were Follicularia of chlorophyta，Monactinus and Aulacoseira of diatom. Redundancy analysis （RDA） of phytoplankton and environmental factors showed that temperature，total nitrogen，pH and nitrate nitrogen were the main environmental factors driving eukaryotic phytoplankton community structure in Danjiangkou Reservoir.In summer，the low diversity of phytoplankton and the high abundance of single dominant species were likely to cause the instability of aquatic ecosystem，so it was suggested to strengthen the awareness of prevention and supervision.

Key words:Danjiangkou Reservoir;high-throughput sequencing;eukaryotic phytoplankton;community structure;driving factor

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