Abstract：With phenol as the sole carbon source,two phenol degrading bacteria designed as WX,JC were isolated after activated sludge taming and culturing from coking plant and municipal waste water treatment plant,respectively. The WX and JC were identified as Achromobacter xylosoxidans NBRC 15126( T) and pseudomonas aeruginosa LMG 1242 Tby physiological and biochemical tests and 16 SrDNA sequence analysis. Then phenol degradation by WX and JC at 40 ℃ were conducted. The results shows that degradation rates of phenol by JC and WX after treatment for 96 h exceeded 90% with initial concentration less than 1 500 mg / L,and reached 43. 17% and 17. 37% with phenol concentration of 1 500 mg / L. Temperatures at 30 ~ 40 ℃,p H value 6 ~ 8,shaking rate of 120 r / min and 7% ~ 16% dosing quantity were found to be the optimum growth condition for phenol degrading bacteria. Phenols in wastewater produced from UCG model test could be completely degraded by WX and JC after treatment for 96 h.

Keyword：underground coal gasification;degradation bacteria;phenol;selection;