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干旱区土壤中西瓜专化型尖孢镰刀菌拮抗菌的筛选及分类
供稿: 耿源濛;孙权;顾欣;王锐;吴宁 时间: 2018-11-14 次数:

作者:耿源濛孙权顾欣;王锐;吴宁

作者单位:宁夏大学农学院

摘要:针对宁夏中部干旱带压砂西瓜田长期连作种植所产生的土壤退化、土壤养分含量降低、土壤真菌性病害增加、西瓜减产、品质下降等问题,从患病的西瓜植株上分离出致病菌-尖孢镰刀菌西瓜专化型(Fusarium oxysporum f.sp.niveum),从宁夏环香山地区压砂瓜集中连片种植区采集健康土壤中分离筛选对西瓜枯萎病致病菌有抑菌效果的拮抗菌株,并对拮抗菌株的防病效果以及分类鉴定进行了研究。通过采用平板稀释法筛选出对目标靶具有拮抗作用的拮抗细菌。通过初筛选取8株对目标靶具有拮抗作用的细菌进一步进行复筛试验。经过数据分析,G-1的抑菌率最大,且与其他处理间存在显著性差异,筛选出对目标靶拮抗最强的拮抗菌为G-1。根据形态学观察、细菌生理生化鉴定和16 S r DNA序列的同源性分析,确定拮抗菌株G-1为萎缩芽孢杆菌(Bacillus atrophaeus)。

基金:宁夏农业特色产业科技支撑项目(2014-43);宁夏重点研发计划项目(nxkjgg2015-06);

关键词:干旱区土壤;西瓜枯萎病;拮抗菌;尖孢镰刀菌;

DOI:10.16186/j.cnki.1673-9787.2016.04.014

分类号:S436.5;S476.1

Abstract:Bottleneck problems such as soil nutrients exhaustion,soil fungal diseases prevalent,soil structure degradation,low production and quality of water melon,already severely restricted Gravel-covered watermelon industry in middle arid area of Ningxia. This experiment is based on the production practice of Grovel-covered Watermellon in Ningxia. Separation of Fusarium oxysporum f. sp. niveum from the incidence of serious disease of watermelon,and the control effect of Fusarium wilt was screened from different types of healthy soil,the effect of antagonistic strain on disease control and classification and identification were studied. During dilution plate method screened with antagonism of antagonistic bacteria to target. Through the screening of 8 strains of bacteria which have antagonistic effect on the target,the screening test was carried out. After data analysis,G-1 inhibitory rate is maximum,and there was a significant difference compared with other treatments,it was screened on the target antagonistic strongest antagonistic to the G-1. According to its morphological,physiological and biochemical characteristics and 16 S r DNA sequence homology analysis can determine the antagonistic strain G-1 is Bacillus atrophaeus.

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